Data Independent Acquisition (DIA)

Data-Independent Analysis (DIA) Proteomics experiments enable deeper proteome coverage, more robust quantitation and reproducible analysis.

In a DIA analysis, all peptides within a defined mass-to-charge (m/z) window are subjected to fragmentation; the analysis is repeated as the mass spectrometer marches up the full m/z range. This results in accurate peptide quantification without being limited to profiling predefined peptides of interest.

Scaffold DIA has a simple workflow for the quantitation of proteins that makes loading and analysing DIA data easy.

Scaffold DIA Intuitive Graphical User Interface enables:

  • Search against FASTA, DIA chromatogram library, BLIB, TraML, or SPTEXT files
  • No need for RT calibration peptides
  • Flexible organization of complex experiments for analysis
  • Delve Into Biological Significance with Gene Ontology to help assess the biological meaning of results ( pie charts and cluster charts)
  • Built-In Statistical Analysis Including PCA
  • Share data with colleagues using Scaffold free Viewer
  • Numerous Excel-formatted data exports and publication-quality images
  • Share data using the free Scaffold DIA Viewer, Excel-formatted exports, and publication-quality images
  • Command Line Interface to automate your DIA experiments
Scaffold DIA sample view

FREE Evaluation

To register for a free 14 day trial of Scaffold or to learn more, please visit the Proteome Software web site – Free Trial.

Further Information

See Downloadable Resources for further technical information or visit Proteome Software Documentation.

Please Contact us if you can not find the information your require or would like a quotation.